2017-8-11 16:0:32025-05-12 18:58:17

locus

BSU36310

BSU_36310

geneLength

339

342

outlinks

bsu

BSU36310

BSU_36310

Gene

Coordinates

3,740,206 → 3,740,547

3,740,206 3,740,547

The protein

Paralogous protein(s)

[[protein|SsbA]]

[[this]]

Biological materials

Mutant

MGNA-A591 (ywpH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/591 NBRP B. subtilis, Japan]

GP3522 (Δ[[gene|ssbB]]::kan), available in [SW|Jörg Stülke]'s lab

MGNA-A591 (ywpH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/591 NBRP B. subtilis, Japan]

BKE36310 ([[gene|ssbB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG

BKK36310 ([[gene|ssbB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG

Biological materials

Expression vector

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

References

Reviews

22933559

22933559, 31950915

References

Original publications

16009133, 17630974, 22373918, 16549871, 14762004, 11948146, 22383849, 22517742, 23536821, 23779106, 25138221, 26707201

16009133, 17630974, 22373918, 16549871, 14762004, 11948146, 22383849, 22517742, 23536821, 23779106, 25138221, 26707201, 31876108

The protein

[SW|Domains]

SSB domain (aa 1-104) (according to UniProt)

Biological materials

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab